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A colorimetric assay for d-Penicillamine in urine and plasma samples based on the aggregation of gold nanoparticles

M. R. Hormozi-Nezhad, M. AzargunN. Fahimi-Kashani 

Journal of the Iranian Chemical Society, 2014, 11, 1249 –1255

We report herein the development of a highly sensitive colorimetric method for the detection of D-Penicillamine using citrate-capped gold nanoparticles (AuNPs). This assay relies upon the distance-dependent of gold nanoparticles' surface plasmon resonance band of gold nanoparticles. By replacing the thiol-containing chelator drug, D-Penicillamine, with citrate on the gold nanoparticles' surface, a new peak appearing at a longer wavelength intensifies and shifts further to the red from the original peak position due to the aggregation of gold nanoparticles which depends on ionic strength, gold nanoparticles and D-Penicillamine concentration. During this process, the plasmon band at 521 nm decreases gradually along with the formation of a new red-shifted band at 630 nm. The calibration curve which is derived from the ratio intensities of absorbance at a longer wavelength (630 nm) to the original wavelength (521 nm) displays a linear relation in the range of 5.0 × 10−6 –3.0 × 10−4 M D-Penicillamine. The lower limit of detection for D-Penicillamine, at the signal-to-noise ratio of 3 (3σ), was 3.8 × 10−6 M. The developed methodology was successfully applied for the determination of D-Penicillamine in human urine and plasma.

Journal Papers
Month/Season: 
January
Year: 
2014

تحت نظارت وف ایرانی

A colorimetric assay for d-Penicillamine in urine and plasma samples based on the aggregation of gold nanoparticles | Nafiseh Fahimi-Kashani

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تحت نظارت وف ایرانی